Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. What is the alternative for ethidium bromide in gel. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Ethidium bromide is typically purchased in powder or solution form and is soluble in water. The fluorescence of ethidium monoazide is weak, but the intensity increases 15fold on binding to dna with excitationemiss.
The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid. Aqueous buffer solutions containing ethidium bromide can be released down the drain after decontamination or destruction. Do not dispose of ethidium bromide as biohazardous waste. The glow dyes are designed to minimize the use of ethidium bromide and the risk associated with the regular use of ethidium bromide. Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair. What is the alternative for ethidium bromide in gel electrophoresis. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet.
It has a role as an intercalator and a fluorochrome. Rna sample loading buffer, with etbr scbt santa cruz. Ethidium bromide etbr is commonly used as a nonradioactive dna stain to iden. Typically eb is purchased in powder or solution form and is soluble in water. Wear gloves and a face mask when working with ethidium bromide in powdered form. Your new online msds binder is a place for you to store the material safety data sheets. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. Thats because the compound is used to make dna fluoresce when running gels, and it does that by slipping neatly betw.
Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution. Rna sample loading buffer, with etbr ethidium bromide, 50gml is formulated for use in rna sample preparation for denaturing formaldehydeagarose gel electrophoresis. The solution can be used to detect both doublestranded and singlestranded dna. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and. The detection limit of dna bound to ethidium bromide is 0. Ethidium bromide, 1% solutionmolecular biology revision date 18jan2018 sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical keep product and empty container away from heat and sources of ignition.
Wipe the area with a damp cloth after the work with ethidium bromide powder is complete. As ethidium bromide is carcinogenic, there are several biological and environmental problems are associated with it. For staining a gel after electrophoresis, dilute a sample of the stock solution to 0. Ethidium bromide is a potent mutagen, often used in laboratories to stain nucleic acids in electrophoresis gels. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as provided for csc1 gradient protocols. After inactivation, the solution, devoid of ethidium bromide,can be poured down the drain with copious amounts of water. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes. Chemical product and company identification section 1. Ethidium has several experimentally useful properties including binding to nucleic acids. Material safety data sheet ethidium bromide solution sdsmsds.
Ethidium bromide safe handling and disposal procedures. Accidental release measures personal precautions wear respiratory protection. Ethidium bromide is a toxic chemical and a mutagen. Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual helix. Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. Ethidium bromide etbr wastes are not regulated by the state of massachusetts or the epa. Ethidium bromide is an intercalating or stacking dye that enters the purine and pyrimidine base pairs. Ethidium bromide is known as an intercalating agent. There is an existing crystal structure in which ethidium bromide is complexed with a ribo cpg dinucleotide.
Environmental precautions prevent further leakage or spillage if safe to do so. Otherwise, aqueous solutions must be deactivated beforedisposal. Safety data sheet creation date 08jun2010 revision date 18jan2018 revision number 4 1. Ethidium bromide is typically in powder or solution form and is soluble in water. Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. Adding ethidium directly to samples prior to loading apr052008 hi everyone, ive read somewhere about adding ethidium directly to the samples to be run on gels prior to running. Load samples and run the gels using your standard protocol. Citations may include links to fulltext content from pubmed central and publisher web sites. Ethidium bromide etbr, a dark red, crystalline, nonvolatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. Are other fluorescent tags used instead of ethidium.
Ive read somewhere about adding ethidium directly to the samples to be run on gels prior to running. The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. The crystal or powder form is odorless and appears dark red in color. It fluorescents a redorange color under ultraviolet uv light and with increased. The flat structure of ethidium bromide allows it to intercalate, or insert, between nitrogenous bases of a dna molecule. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. Ethidium bromide laboratory spill and waste handling procedures. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. Bp210 synonyms no information available recommended use laboratory chemicals.
Pubmed comprises more than 26 million citations for biomedical literature from medline, life science journals, and online books. Citations may include links to fulltext content from pubmed central and. Ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. Ethidium bromide is strongly mutagenic, causing living cell mutations. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as. It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. Liquid ethidium bromide waste can be collected in the same container as the solid waste. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. A safer alternative is to purchase ethidium bromide in solution. It fluoresces a redorange colour under ultraviolet light and with increased fluorescence when bound to doublestranded dna. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. Ethidium bromide fact sheet environmental health and safety. Ask most biologists about handling it, and youre get a fearful expression and advice to use gloves, etc.
Standard operating procedure template for ethidium bromide. Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. This eliminates the hazards of working with the powdered form. Safety data sheet northwest missouri state university. Sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Handling when pure ethidium bromide is used, handling should be performed in a fume hood wearing full protection clothing including a lab coat, closedtoe shoes, chemical resistant gloves and chemical safety goggles. No warranty, express or implied, is made and labchem inc. Please follow the instructions listed in this fact sheet when handling etbr. Product name ethidium bromide, 1% solutionmolecular biology cat no. Ethidium bromide solution, molecular grade 10mgml, is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. That said, when mum brought back some veinasbestos samples from. The more common use of the ethidium bromide stain is the detection of dna or rna gel samples. Dec 19, 20 ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis.
Ethidium bromide is used to visualize dna in agarose gels and in many cesium chloride gradient protocols. Ethidium bromide safe handling, disposal, and emergency procedures. But a msds for sybr safe reports a ld50 for rats of 5gkg, which is higher. The user must determine suitability of this information for his application. Bring information safety data sheet about ethidium bromide to medical center with you.
The powder form is considered an irritant to the upper respiratory track, eyes and skin. Ethidium is the fluorescent compound widely used in experimental cell biology and biochemistry to reveal doublestranded dna and rna. Due to its unique structure, it can easily intercalate into dna strand. Glow dyes have been specifically formulated for nucleic acid. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy.
Ethidium reemits this energy as yelloworange light centered at 590 nm. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm. Material should be handled according to the material safety data sheet msds. I am trying to minimize background while having nice sharp bands for microarray rtpcr validations. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required.
Exposure information commercial availabili production and producers. A uv light can be used to determine complete inactivation. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes. The powder is used to make a stock solution of 10 mgml, and the working concentration for gelstaining is 1 gml. Apr 18, 2016 ethidium bromide is found in pretty much every molecular biology lab around. Ethidium bromide is not regulated as hazardous waste at low concentrations, but is treated as hazardous waste by many organizations. Ethidium bromide is found in pretty much every molecular biology lab around. Ethidium bromide is a potent mutagen used as a nucleic acid stain. The disposal of laboratory ethidium bromide remains a controversial subject. Are other fluorescent tags used instead of ethidium bromide.
These filters use ionexchange resins and activated charcoal to remove the ethidium bromide from. Ethidium bromide solution 10 mgml catalog or product number. Ethidium bromide solution sc286960 hazard alert code key. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated dye. Material safety data sheet section 1 ethidium bromide 1 chemical product and company identification msds name. Ethidium bromide waste disposal fact sheet environmental. Eb was reported by watkins 1952 to be prepared from 3. Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t. It is preferable to use ecofriendly flourescent dyes such as. View the stained gel using a standard transilluminator 302 or 312 nm and image the gel using an ethidium bromide filter. Ethidium bromide solutions and buffer solutions containing ethidium bromide should be filtered, decontaminated, or destroyed using one of the methods below or collected as chemical waste.